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In this study, a more conventional approach was used to analyze the effects of the population of species in the skin microbiome. The effect of the microbial population present on the skin with the application of three antimicrobial peptides [Leuconostoc radish root ferment filtrate, Lactobacillus ferment, and Lactobacillus & Cocos nucifera (coconut) fruit extract] was compared with a negative control (water) and a positive
control (triclosan). The microbiome population was determined by DNA extraction, 16S ribosomal RNA (rRNA) polymerase chain reaction (PCR) amplification, and sequencing.

In the original scientific publication evaluating sunscreen methodologies, Garzarella and Caswell showed there to be no clinically significant or statistically significant difference in the average Sun Protection Factor (SPF) of a sunscreen formulation between any of three methodologies, Food and Drug Administration (FDA) Final Monograph, Australia\/New Zealand, and European Cosmetics Association (COLIPA) International, suggesting that any differences in methodology were insignificant in the resulting SPF determined.