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Determination of Fluorescent Whitening Agents in Cosmetics and Liquid Detergent by High-Performance Liquid Chromatography with Diode Array Detector in Tandem with Fluorescence Detector

Journal of Cosmetic Science | Vol. 69 No. 4
Authored by Yu-Xiang Gu, Jin-Qin Yang, Si-Yi Pan, Lei Wang, and Ze-Lin Zhou


Five distyryl-type fluorescent whitening agents (FWA85, 210, 220, 351, and 353) were determined in cosmetics and liquid detergent by high-performance liquid chromatography with diode array detector in tandem with fluorescence detector. The samples were extracted with ultrasound in 33% acetonitrile for 10 minutes and the components were determined by ion-pair chromatography on an MG C18 column. The limits of detection were from 0.01 to 0.1 mg·kg −1 and the limits of quantification were from 0.04 to 0.4 mg·kg −1 . The recovery was from 80.7 to 103.3%. A linear relationship was present from 0.10 to 100 µg·ml −1 of FWAs. The protocol was simple, sensitive, selective, and was successfully applied to analyze distyryl-type FWAs in cosmetics and liquid detergent. FWA351 and FWA85 were detected in several samples with the concentrations of 19.4–1,130 mg·kg −1 .

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