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Simultaneous Determination of Nine Sunscreen Agents by HPLC and Chemometric Analysis

Journal of Cosmetic Science | Vol. 70 No. 4
Authored by Fatemeh Moraffah, Hanieh Adli, Akram Rostami, Mohammad R. Khoshayand, Mohsen Amini, Zeinab Pourjabbar, Sareh Kargar, Naficeh Sadeghi, and Mannan Hajimahmoodi


A worldwide outbreak of skin cancer, related to ultraviolet (UV) radiations, was reported. Therefore, primary prevention programs were initiated. Application of sunscreens is one of the most efficient ways of protection; however, their efficiency and safety have remained a challenging issue. So, it seems necessary to consider the potential side effects for limiting the use and amount of sunscreens. In this study, an high performance liquid chromatography (HPLC) system equipped with a UV–visible detector has been used. For separation, an Agilent C 18 column was used (Agilent Technologies, Santa Clara, CA). This method was applied for quantitative determination of nine UV filters in commercial sunscreen products which were widely used in Iran. Fifty samples of Iranian and imported sunscreen products were analyzed. The detection limit was determined to be 0.439–1.481 µg\/ml, and the quantization limit was determined to be 1.330–4.490 µg\/ml. Also, in this study, chemometric methods were used to investigate the differences between Iranian and other countries’ sunscreen brands. It was observed that despite the amount of UV filters in Iranian sunscreens, which was in the allowed range, there were some differences between Iranian and other countries’ sunscreens. The proposed HPLC method allows efficient and simultaneous analysis of UV filters and is suitable as a quality control assay for commercial sunscreen products.


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